Novel developments of in vivo haploid technologies in vegetables
(contact: Prof. Laurent GENTZBITTEL,, Industrial PhD under discussion)

The development of double haploids significantly reduced the time to obtain pure lines and as such, speed up the plant breeding program. However, there are no efficient methods for double haploidisation in onion, a major condiment and vegetable in Russian cuisine.

The main goal of the project is to implement a recent disruptive agrobiotechnology method based on the genome editing of the centromeric histone CENH3 which opens a new avenue for the generation of haploid-inducing lines.

The specific goals will be:

1. To develop a protocol for centromere-mediated genome elimination method by altering CENH3 gene(s) in onion, using CRISPR/Cas9 or Cpf1

2. To create diploid, transgene-free, genome-edited, haploid inducer cultivars of onion having altered CENH3 gene

3. To evaluate the performances of these haploid inducer lines by carrying out crosses with wild-type plants and evaluating the rates in producing haploid embryos

Required skills: Plant biotechnology, molecular biology (incl. cloning), plant biology, basics of bioinformatics (blast, primer design).

Deadline for application: April 15, 2024