(Contact: Prof. Cécile BEN , C.Ben@skoltech.ru )
The development of double haploids significantly reduced the time to obtain pure lines and as such, speed up the plant breeding program. However, there are no efficient methods for double haploidisation in sunflower and sugar beet.
The main goal of the project is to implement a recent disruptive agrobiotechnology method based on the genome editing of the centromeric histone CENH3 which opens a new avenue for the generation of haploid-inducing lines.
The specific goals will be:
1. To develop a centromere-mediated genome elimination method by altering CENH3 gene(s) in sugar beet and sunflower, using CRISPR/Cas9 or Cpf1
2. To create diploid, transgene-free, genome-edited, haploid inducer cultivars of sugar beet and sunflower having altered CENH3 gene
3. To evaluate the performances of haploid inducer lines by carrying out crosses with wild-type plants and evaluating the rates in producing haploid embryos
Skills: Plant biotechnology, molecular biology (incl. cloning), plant biology, basics of bioinformatics (blast, primer design).
You must apply through the entire Skoltech PhD admissions process in order to be considered for the aforementioned PhD position.
Deadline: Sunday, July31, 2022